--> -->

Ossiform​ initiates new cancer model research project

Optimizing P3D Scaffolds based bone tumor models ― for cancer research on metastases to bone

Ossiform is happy to welcome our new research intern, Rie Andersen Lassen, who will be engaging in a new cancer model research project to optimize P3D Scaffolds-based tumor models for research on metastases to bone. That is, the spread of cancer cells from cancerous tumors to bone.


Below, you can read how Rie describes her project.

The aim of this project is to optimize the P3D Scaffold for application in the field of bone metastases research to construct in vitro models suitable as replacements for in vivo studies to reduce animal harm and study costs.


The optimization will be focused on improving the composition of the P3D Scaffolds’ components and structure, paired with human mesenchymal stem cells (hMSCs) to construct a “base” scaffold that exhibit traits and transmitter substance comparable to native bone tissue.


Bone formation and transmitter substance determination will be used to determine if an optimized model has been reached compared to cell well plates and the original P3D Scaffold. The models and comparators are then to be introduced to co-cultures of normal hMSCs and cancer cell lines to construct tumoroid models that will then be subjected to chemotherapeutic treatment.


The effects of the chemotherapeutic responses are measured and compared across sample groups. The results analyzed from the tumoroid models will be compared to literature.


Rie Andersen Lassen
​Research Intern, Biomedicine

Background to the study

Bone metastasis, which is a transfer of cancer from solid tumors to bone, commonly originates from thyroid, gynecologic, breast, colorectal, lung, prostate, and melanoma [1].

The two most frequent cancer types that metastases to bone originate from is breast cancer and prostate cancer.

Unfortunately, there is currently no effective treatment once the tumor cells have metastasized to bone. Finding treatments of metastases to bone is therefore a major challenge in the field of research [1, 2].

One of the problems in this research is the design of a proper bone marrow model , especially because bone marrow has a very complex 3D microenvironment [2].

Cancer models for research on metastasis to bone

To date, models used for research on metastasis to bone have typically exploited animal models or 2D cell cultures using human cell lines.

However, animal models differ in physiology and metabolism between species and 2D cell cultures of human cell lines cannot imitate the 3D microenvironment of metastases in bone [3, 4].

Meanwhile, organoids present a promising alternative or supplement as these models maintain cell-to-cell contact by providing a 3D environment for the natural expansion of cells [3].

Thereby, organoids may overcome some of the challenges that 2D cell cultures and animal models (such as Caenorhabditis eleangans, Drosophila melanogaster and Mus musculus) faces. Still, this depends on the construct of the human organoid.

Introducing organoids in cancer research

For most human organoids, the advantages are that they are:

  • human-derived (representing the human physiology in contrast to 2D cell cultures using human cell lines);
  • and relatively easy to genetically manipulate in combination with induced pluripotent stem cells.
  • Finally, organoids are less costly than mouse models but are relatively costly compared to yeast models, fly models, and traditional two-dimensional cell lines [5].

To some extent, the aim of human organoids usage is to limit the usage of animals for testing – by utilizing the physiological features of the technology.

However, unfortunately, human organoids cannot entirely exclude the use of animal models as animals include molecular pathways and metabolism that organoids are unable to promote [3, 5].

Overall, organoids open the opportunity for more complex studies than 2D cell cultures while limiting the usage of animals in research of the pathophysiology of disease and the development of drugs and personalized medicine [5].

An organoid model that is under development is the the combination of hMSCs and the P3D Scaffold by Ossiform, which mimics the mineralized bone environment and can be used to grow tumoroids for cancer research on metastases to bone [6].

If you have any questions, please email research@ossiform.com.

References

  1. Fornetti, J., A.L. Welm, and S.A. Stewart, Understanding the Bone in Cancer Metastasis. J Bone Miner Res, 2018. 33(12): p. 2099-2113.
  2. Maurizi, A. and N. Rucci, The Osteoclast in Bone Metastasis: Player and Target. Cancers (Basel), 2018. 10(7).
  3. Scheinpflug, J., et al., Journey into Bone Models: A Review. Genes (Basel), 2018. 9(5).
  4. Fontoura, J.C., et al., Comparison of 2D and 3D cell culture models for cell growth, gene expression and drug resistance. Mater Sci Eng C Mater Biol Appl, 2020. 107: p. 110264.
  5. Kim, J., B.K. Koo, and J.A. Knoblich, Human organoids: model systems for human biology and medicine. Nat Rev Mol Cell Biol, 2020. 21(10): p. 571-584.
  6. Wellejus, M. Miniature tumors can be grown on P3D scaffolds for cancer research. 2020 Oct 21, 2020.

Our mission is your benefits

Our mission is to provide 3D printed natural bone implants to reduce complications, improve functional & aesthetic outcomes, and obtain faster recovery.

Natural bone porosity, optimized for bone regeneration 

The unique structure of the P3D Bone is designed to facilitate the natural forming of new bone.

Remodels into real living bone

The natural material and structure ensure effective remodeling of the implant into new vascularized bone.

Patient specific - designed with the patient's uniqueness in mind

The P3D Bone is 3D printed to enable a full restoration of the functionality and appearance of bones.

Resorbable material with structural support

P3D Bone eliminates the need to harvest bone as well as the need for permanent and ill-fitting implants.

P3D Bone Void Filler is expected to launch in 2023

Our first 3D printed, resorbable P3D Bone with modifiable dimensions and a lifelike bone architecture

P3D Bone Patient Specific Implant is expected to launch in 2024

Our patient specific and resorbable P3D Bone based on the patient’s own CT/MRI scan data

We Print Bone​™

Contact us here

Contact us for questions, general inquiries, or to request a quote.
We strive to provide you with highest quality service and expertise in medical 3D printing.

 
 
 
Please make sure all required fields are filled out correctly

Do you have questions?

Give us a call or write to us by email: info@ossiform.com

We will get back to you as soon as possible.​

logo-footer_2

Ossiform ApS - We Print Bone™

​Oslogade 1, 5000 Odense C​, Denmark

CVR. 38838512

facebook  linkedin  twitter  instagram