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News from the Lab

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A study aimed to investigate the behavior of human-derived osteoclasts cultured on a P3D Scaffold

June 2, 2023 | News from the Lab

CD14+ monocytes were isolated from blood of human donors and subsequently differentiated into mature osteoclasts. The mature osteoclasts were cultured on rhodamine (red) stained P3D Scaffolds and f-actin (green) in living osteoclasts was labeled using SiR-actin.

A time-lapse was conducted over a period of 70 hours with images acquired every 21 minutes. By employing time-lapse microscopy, it was possible to confirm osteoclast resorption of the scaffold.

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Ossiform and Finnadvance announce partnership

April 19, 2023 | News from the Lab

We are thrilled to announce that Finnadvance Ltd. ̶ a Finnish biotechnology company developing highly scalable organ-on-chip platforms ̶ has published a whitepaper in partnership with Ossiform.

This whitepaper explores the various prospects of reconstructing microphysiological conditions of tissues and biological interfaces in a scalable fashion, by employing microfluidics and combining the microfluid properties of AKITA® Plate with P3D Scaffolds from Ossiform.

Under the partnership between Ossiform and Finnadvance, the P3D Scaffolds is incorporated into the AKITA Plate. This combined platform will permit the test of bone repair studies in a high-throughput compatible manner while the flow of the Akita system increases the signal-to-noise ratio up to 5 times.

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Comparing P3D Scaffolds to slices of real bone

March 31, 2023 | News from the Lab

As part of a larger study, we have compared our P3D Scaffolds to slices of bone. Here, we found that there is no significant difference in cell viability after 28 days between slices of bone and the P3D Scaffolds, as it appears on the graph in the upper left corner. Furthermore, it is clear that the variance within the test-groups is much lower in the P3D Scaffolds than bone slices.

The SEM images show osteoblast produced collagen on a P3D Scaffold (to the right) and a bone slice (to the left).

Combined with the scaffolds’ physical resemblance with bone in terms of calcium phosphate content, porosity and stiffness, the P3D Scaffolds are an obvious alternative to traditional cell culturing and hydrogel-based 3D cell culture systems.

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Technical data sheet available for P3D Scaffolds

December 20, 2022 | News from the Lab

This year, we have been busy in the lab fine tuning the small details and testing various methods and techniques in combination with our P3D Scaffolds.

Now we can share that all the testing has been boiled down to our first technical data sheet! Here you will find general guidelines, relevant flow charts, and links for all our protocols and other materials for researchers.

Download technical data sheet

P3D Scaffolds support normal morphology of osteoblasts

October 27, 2022 | News from the Lab

Our latest studies have shown that the P3D Scaffolds have great osteogenic properties and that osteoblasts maintain their genotype and morphological phenotype. We have had the osteoblasts cultured for 2-5 weeks and the results are clear.

Osteoblastic marker genes are upregulated in response to traditional cell culturing. Confocal imaging shows how the P3D scaffolds allow the osteoblasts to organize in a 3D oriented manner, surrounded by their collagen-rich extracellular matrix.

Studying the osteoblasts and their extracellular matrix closer using scanning electron microscopy, you can really appreciate how the osteoblasts are retaining their round morphology, and how the extracellular matrix is spanning widely across the scaffold clearly indicative of the first steps in bone formation.

Osteoblasts living in culture on P3D Scaffolds for 20 weeks

September 15, 2022 | News from the Lab

Even though our material is fully resorbable both in vivo and in vitro, it actually does not spontaneously dissolve while being used for cell culturing.

Our scientists have been testing it for weeks, and now we are ready to give a brief status update on our long term culturing experiment.

Currently, we have had osteoblasts in culture on P3D Scaffolds for 20 weeks. On the image to the left you’ll see a 10x magnification of osteoblasts residing in their extracellular matrix within a macropore of the P3D Scaffolds. To the right you’ll see that the P3D Scaffolds are still fully intact.

Judging by the light microscope images, the cells are thriving and showing that long term culturing of osteoblast for bone formation studies are easy to achieve using P3D Scaffolds.

New master's thesis project in collaboration with Ossiform

July 19, 2022 | News from the Lab

At the end of summer, Laura will be starting her master's thesis project here at Ossiform. She is currently studying for a Master of Science in Chemical Engineering at the University of Southern Denmark.

Using our P3D Scaffolds, Laura will investigate potential antibacterial solutions and how they may be combined with our proprietary technology. Laura aims to develop a 3D printed bioceramic scaffold which minimizes the risk of biofilm formation through a constant release of antibacterial factors.

We cannot wait to share her results with you.

Collagen-producing osteoblasts

May 31, 2022 | News from the Lab

The development of our bones is carried out by the bone-forming cells called osteoblasts. Through a process known as osteogenesis, the osteoblasts form the bone by producing a soft framework, consisting primarily of collagen, which is then strengthened and hardened by the addition of calcium phosphate. The combination of the flexible collagen and the hard calcium phosphate is what makes our bones sturdy enough to withstand loadbearing and stress.

Collagen production is therefore a good initial measure of bone forming osteoblast activity. These confocal images of osteoblasts cultured on P3D Scaffolds show the osteoblasts (in blue) and the surrounding osteoblast-produced collagen (in green), illustrating that the 3D cell culture system supports the bone-forming process.

If you want to learn more about the P3D Scaffolds, please visit our Resources page or book a free research consultation with our Scientific Team:

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Upregulated expression of the alkaline phosphatase gene

April 28, 2022 | News from the Lab

Alkaline phosphatase plays a vital role in osteogenesis and bone formation and is one of three identified osteogenic marker genes. At Ossiform, we are happy to show new preliminary data which indicates that osteoblasts cultured on the P3D Scaffolds have an upregulated expression of the alkaline phosphatase gene when compared to traditional cell culturing.

These data suggests that the P3D Scaffolds are beneficial for the osteogenic program in vitro on a transcriptional level, thereby supporting their natural genetic fate.

Want to discuss how you can use the 3D cell culture systems in your study? Book an online research consultation with our Scientific Team to learn more and request your sample.

New scientific paper on Ossiform's P3D Bone

March 14, 2022 | News from the Lab

In this newly published paper, Ossiform has compared its 3D printed P3D Bone implant with an off-the-shelf b-TCP implant. The implants were inserted in the mandible ramus of pigs, and after six months, resorption and remodeling were evaluated using CT-scans and histological images.

After six months, the P3D Bone implants were almost completely resorbed and replaced by vascularized compact bone, allowing osteointegration to occur. The histological image in the upper right corner shows the partially resorbed P3D Bone implant only three months after implantation.

The full results supported by histology and CT data are published in Bone, Volume 159, June 2022.

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Fresh from the Scanning Electron Microscope

March 3, 2022 | News from the Lab

Earlier this week, our in-house scientist, Nanna, used SEM imaging to evaluate if osteoblasts cultured on the P3D Scaffolds are producing collagen. This led to several intriguing images that show a large amount of collagen produced.

The picture shown in this weeks "News from the Lab" illustrates how the P3D Scaffolds allow osteoblasts to produce an extra cellular matrix that spans throughout the scaffold in an in vivo like manner.

If you want to learn more about culturing of cells on our P3D scaffolds, please visit our resources page.

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