Protocols and guidelines​

​Download our protocols and guides on how to make 3D cell cultures, using the P3D Scaffolds.

If you have any questions, email We are always available to discuss how we can help your team develop the right 3D cell model customized to your research needs.​

P3D Scaffold
Cell Recovery by Trypsination

This protocol explains how to retrieve cells that have been cultured on the P3D Scaffolds. Cell recovery from the P3D Scaffolds can be performed using standard protocols with minor modifications.

How to 3D Culture Human Mesenchymal Stem Cells

This protocol is for seeding and 3D culturing stem cells on P3D Scaffolds in a 24-well plate. The procedure provides guidelines on how to create three-dimensional multicellular in vitro tissue constructs based on hMSCs and subsequently differentiate the hMSCs into osteoblasts to create a 3D bone cell culture.

How to Verify Osteogenesis on P3D Scaffolds

This guide is for studying osteoblast differentiation using an ALP staining assay. Mesenchymal stem cells express alkaline phosphatase (ALP), which increases during osteoblast differentiation. ALP levels can therefore be used as an osteogenic marker.

Do you want to know more about applications and use cases of the 3D cell culture system?

​P3D Scaffolds were recently utilized as tissue engineering scaffolds in an in vivo study. 

The purpose of the experiment was to test and develop a system for non-invasive, non-destructive, and label-free monitoring of bone tissue development, using novel spectroscopic techniques.

The material and customized shape of the P3D Scaffolds provided a clinically relevant system that allowed subcutaneous implantation in mice, while supporting osteogenic differentiation of mesenchymal stem cells.

Our mission is your benefits

Our mission is to provide 3D printed natural bone implants to reduce complications, improve functional & aesthetic outcomes, and obtain faster recovery.

Natural bone porosity, optimized for bone regeneration 

The unique structure of the P3D Bone is designed to facilitate the natural forming of new bone.

Remodels into real living bone

The natural material and structure ensure effective remodeling of the implant into new vascularized bone.

Patient specific - designed with the patient's uniqueness in mind

The P3D Bone is 3D printed to enable a full restoration of the functionality and appearance of bones.

Resorbable material with structural support

P3D Bone eliminates the need to harvest bone as well as the need for permanent and ill-fitting implants.

P3D Bone Void Filler is expected to launch in 2022

Our first 3D printed, resorbable P3D Bone with modifiable dimensions and a lifelike bone architecture

P3D Bone Patient Specific Implant is expected to launch in 2024

Our patient specific and resorbable P3D Bone based on the patient’s own CT/MRI scan data

We Print Bone​™

Contact us here

Contact us for questions, general inquiries, or to request a quote.
We strive to provide you with highest quality service and expertise in medical 3D printing.

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Ossiform ApS - We Print Bone™

​Oslogade 1, 5000 Odense C​, Denmark

CVR. 38838512

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