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Protocols and guidelines​

​Download our 3D cell culture protocols and guides here

If you have any questions, email research@ossiform.com. We are always available to discuss how we can help your team develop the right 3D cell model customized to your research needs.​

P3D Scaffold
Cell Recovery by Trypsination

This protocol explains how to retrieve cells that have been cultured on the P3D Scaffolds. Cell recovery from the P3D Scaffolds can be performed using standard protocols with minor modifications.

How to Culture Human Mesenchymal Stem Cells in Bioceramic 3D Cell Culture Systems

This protocol is for seeding and 3D culturing stem cells on P3D Scaffolds. The procedure provides guidelines on how to create three-dimensional multicellular in vitro tissue constructs based on hMSCs and subsequently differentiate the hMSCs into osteoblasts to create a 3D bone cell culture.

How to Verify Osteogenesis on P3D Scaffolds

This guide is for studying osteoblast differentiation using an ALP staining assay. Mesenchymal stem cells express alkaline phosphatase (ALP), which increases during osteoblast differentiation. ALP levels can therefore be used as an osteogenic marker.

​More Technical Resources

Visit Ossiform's YouTube channel to view our video protocols​

Download the P3D Scaffolds Product Promotion File​

Satefy Data Sheets​

Here we provide the Safety Data Sheets (SDS) for our P3D Scaffolds products. The SDS provides information on chemical products that helps users make risk assessments, and gives information on handling, storage, and emergency measures in case of accident.​

Do you want to know more about applications and use cases of the 3D cell culture system?

​P3D Scaffolds were recently utilized as tissue engineering scaffolds in an in vivo study

The purpose of the experiment was to test and develop a system for non-invasive, non-destructive, and label-free monitoring of bone tissue development, using novel spectroscopic techniques.

The material and customized shape of the P3D Scaffolds provided a clinically relevant system that allowed subcutaneous implantation in mice, while supporting osteogenic differentiation of mesenchymal stem cells.

We Print Bone​™

Contact us here

Contact us for questions, general inquiries, or to request a quote.
We strive to provide you with highest quality service and expertise in medical 3D printing.

 
 
 
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Give us a call or write to us by email: info@ossiform.com

We will get back to you as soon as possible.​

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Ossiform ApS - We Print Bone™

​Oslogade 1, 5000 Odense C​, Denmark

CVR. 38838512

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