Protocols and guidelines

View our 3D cell culture
protocols and video guides

If you have any questions, email our technical team at research@ossiform.com. We are always available to discuss how we can help you develop the best model for your research needs.​

See also our Frequently Asked Questions →

How to culture hMSCs via bioceramic 3D cell culture systems

This guide is for seeding and 3D culturing stem cells on P3D Scaffolds. The procedure provides guidelines on how to create 3D multicellular in vitro tissue constructs based on hMSCs and subsequently differentiate the hMSCs into osteoblasts to create a bone cell culture.

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How to verify osteogenesis on P3D Scaffolds

This guide is for studying osteoblast differentiation using an ALP staining assay. Mesenchymal stem cells express alkaline phosphatase (ALP), which increases during osteoblast differentiation. ALP levels can therefore be used as an osteogenic marker.

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How to perform cell recovery by trypsinization

This protocol explains how to retrieve cells that have been cultured on the P3D Scaffolds. Cell recovery can be performed using standard protocols with an additional centrifugation step to ensure that any cells trapped within the scaffold are retrieved.

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How to use Invitrogen LIVE/DEAD Cell Imaging Kit with P3D Scaffolds

This protocol explains how to perform Cell viability using Invitrogen LIVE/DEAD Cell Imaging Kit with P3D Scaffolds.

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Protein extraction when using P3D Scaffolds

This protocol explains how to perform protein extraction when using P3D Scaffolds.

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Evaluation of cell viability ― using CellTiter-Glo 3D Cell Viability Assay

This protocol explains how to evaluate cell viability using CellTiter-Glo 3D cell viability assay with P3D Scaffolds.

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Harvesting RNA from cells cultured on P3D Scaffolds

This protocol explains how to harvest RNA from cells cultured on P3D Scaffolds.

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More Technical Resources

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Figure that shows a selection of standard analysis methods that are compatible with P3D Scaffolds, including light and confocal microscopy, SEM, transciptomics, raman spectroscopy ,and quantitative PCR

Download our P3D Scaffolds Technical Data Sheet

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Ossiform scientists working in the laboratory, wearing white lab coats and green gloves.

Download our P3D Scaffolds (RUO) Safety Data Sheet

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Researcher studies cultured P3D Scaffolds under a microscope.

Do you want to know more about applications and use cases of the 3D scaffolds?

P3D Scaffolds have been used in a variety of studies, including as tissue engineering scaffolds in an in vivo study.

The purpose of the experiment was to test and develop a system for non-invasive, non-destructive, and label-free monitoring of bone tissue development, using novel spectroscopic techniques.

The material and customized shape of the P3D Scaffolds provided a clinically relevant system that allowed subcutaneous implantation in mice, while supporting osteogenic differentiation of mesenchymal stem cells.

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