CD14+ monocytes were isolated from blood of human donors and subsequently differentiated into mature osteoclasts. The mature osteoclasts were cultured on rhodamine (red) stained P3D Scaffolds and f-actin (green) in living osteoclasts was labeled using SiR-actin.

A time-lapse was conducted over a period of 70 hours with images acquired every 21 minutes. By employing time-lapse microscopy, it was possible to confirm osteoclast resorption of the scaffold.